Composite

Part:BBa_K4165074:Design

Designed by: Hossam Hatem   Group: iGEM22_CU_Egypt   (2022-09-30)


T7 promoter-6x His tag-TRIM21-DocS-T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 311
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

this sequence was optimized to be expressed in E. coli.


Source

this sequence was retrieved from literature.

References